Journal: Cells

Article Title: Aberrantly Over-Expressed TRPM8 Channels in Pancreatic Adenocarcinoma: Correlation with Tumor Size/Stage and Requirement for Cancer Cells Invasion

doi: 10.3390/cells3020500

Figure Lengend Snippet: Immunohistochemistry using anti-TRPM8 in pancreatic tissues. ( A ) Normal pancreatic tissues. ( B ) Chronic pancreatitis. ( C ) Pancreatic intra-epithelial neoplasm. ( D ) Intraductal papillary mucinous neoplasm.

Article Snippet: Four plasmids containing different shRNA directed against human TRPM8 and a plasmid containing non-targeting shRNA were obtained from Superarray Biosciences/Qiagen (Valencia, CA, USA) and tested for efficiency of gene silencing.

Techniques: Immunohistochemistry

Journal: Cells

Article Title: Aberrantly Over-Expressed TRPM8 Channels in Pancreatic Adenocarcinoma: Correlation with Tumor Size/Stage and Requirement for Cancer Cells Invasion

doi: 10.3390/cells3020500

Figure Lengend Snippet: Immunohistochemical analysis of TRPM8 in malignant pancreatic tumors. ( A , B ) Adenocarcinoma. ( C , D ) Adenosquamous carcinoma. ( E , F ) Solid pseudo-papillary neoplasm ( G , H ) Acinar cell carcinoma. ( I , J ) Neuroendocrine tumor. ( A , C , E , G , I ) H and E, original magnification ×200. ( B , D , F , H , J ) Immunohistochemistry using anti-TRPM8 antibodies, original magnification ×400.

Article Snippet: Four plasmids containing different shRNA directed against human TRPM8 and a plasmid containing non-targeting shRNA were obtained from Superarray Biosciences/Qiagen (Valencia, CA, USA) and tested for efficiency of gene silencing.

Techniques: Immunohistochemical staining, Immunohistochemistry

Journal: Cells

Article Title: Aberrantly Over-Expressed TRPM8 Channels in Pancreatic Adenocarcinoma: Correlation with Tumor Size/Stage and Requirement for Cancer Cells Invasion

doi: 10.3390/cells3020500

Figure Lengend Snippet: Expression of TRPM8 in various types of histopathology in pancreatic tumors with regard to the intensity of immunoreactivity and the percentage of positive cells. The total number of cases examined is 308. The values represent the number of specimens with the corresponding proportions in parentheses.

Article Snippet: Four plasmids containing different shRNA directed against human TRPM8 and a plasmid containing non-targeting shRNA were obtained from Superarray Biosciences/Qiagen (Valencia, CA, USA) and tested for efficiency of gene silencing.

Techniques: Expressing, Histopathology

Journal: Cells

Article Title: Aberrantly Over-Expressed TRPM8 Channels in Pancreatic Adenocarcinoma: Correlation with Tumor Size/Stage and Requirement for Cancer Cells Invasion

doi: 10.3390/cells3020500

Figure Lengend Snippet: Expression levels of TRPM8 in pancreatic adenocarcinoma. Images in ( A , C , E ) (original magnification ×200) represent the H&E sections of the same tumor as the TRPM8 immunohistochemical staining in ( B , D , F ) (original magnification ×400), respectively. Expression levels of TRPM8: ( B ) no-to-low; ( D ) moderate; ( F ) high.

Article Snippet: Four plasmids containing different shRNA directed against human TRPM8 and a plasmid containing non-targeting shRNA were obtained from Superarray Biosciences/Qiagen (Valencia, CA, USA) and tested for efficiency of gene silencing.

Techniques: Expressing, Immunohistochemical staining, Staining

Journal: Cells

Article Title: Aberrantly Over-Expressed TRPM8 Channels in Pancreatic Adenocarcinoma: Correlation with Tumor Size/Stage and Requirement for Cancer Cells Invasion

doi: 10.3390/cells3020500

Figure Lengend Snippet: Anti-TRPM8 immunoreactivity in pancreatic adenocarcinoma positively correlates with primary tumor size and stages. The distribution of the IHC score for expression of TRPM8 is displayed against age/gender, histological grade, primary tumor (T), and stage. The total number of specimens of pancreatic adenocarcinoma examined is 280. Polyserial correlation between the primary tumor size (T) or the tumor stages and the log-transformed IHC score is 0.13 and 0.10, respectively.

Article Snippet: Four plasmids containing different shRNA directed against human TRPM8 and a plasmid containing non-targeting shRNA were obtained from Superarray Biosciences/Qiagen (Valencia, CA, USA) and tested for efficiency of gene silencing.

Techniques: Expressing, Transformation Assay

Journal: Cells

Article Title: Aberrantly Over-Expressed TRPM8 Channels in Pancreatic Adenocarcinoma: Correlation with Tumor Size/Stage and Requirement for Cancer Cells Invasion

doi: 10.3390/cells3020500

Figure Lengend Snippet: The percent coverage ± standard error for expression of TRPM8 in pancreatic adenocarcinoma with respect to the intensity and the tumor stage. The numbers in parentheses represent the patient counts. NA, not applicable.

Article Snippet: Four plasmids containing different shRNA directed against human TRPM8 and a plasmid containing non-targeting shRNA were obtained from Superarray Biosciences/Qiagen (Valencia, CA, USA) and tested for efficiency of gene silencing.

Techniques: Expressing

Journal: Cells

Article Title: Aberrantly Over-Expressed TRPM8 Channels in Pancreatic Adenocarcinoma: Correlation with Tumor Size/Stage and Requirement for Cancer Cells Invasion

doi: 10.3390/cells3020500

Figure Lengend Snippet: Over-expression of TRPM8 protein in human pancreatic adenocarcinoma cell lines. ( A ) The protein levels of TRPM8 in H6c7, MIA PaCa-2, PANC-1, and BxPC-3 cells were determined using immunoblotting with anti-TRPM8 antibodies. The GAPDH protein levels were analyzed as internal controls. ( B ) The relative protein levels of TRPM8 are expressed as % (mean ± standard error) of that in H6c7.

Article Snippet: Four plasmids containing different shRNA directed against human TRPM8 and a plasmid containing non-targeting shRNA were obtained from Superarray Biosciences/Qiagen (Valencia, CA, USA) and tested for efficiency of gene silencing.

Techniques: Over Expression, Western Blot

Journal: Cells

Article Title: Aberrantly Over-Expressed TRPM8 Channels in Pancreatic Adenocarcinoma: Correlation with Tumor Size/Stage and Requirement for Cancer Cells Invasion

doi: 10.3390/cells3020500

Figure Lengend Snippet: Short hairpin RNA-mediated silencing of TRPM8 impaired invasion of pancreatic adenocarcinoma cells. BxPC-3 and MIA PaCa-2 cells transfected with either anti- TRPM8 shRNA or NC shRNA were analyzed for cell invasion using the trans-well assay. Cell invasion is expressed % (mean ± standard error) of that in the cells transfected with control shRNA. Representative images of the invaded cells stained with crystal violet are shown at 200× magnification.

Article Snippet: Four plasmids containing different shRNA directed against human TRPM8 and a plasmid containing non-targeting shRNA were obtained from Superarray Biosciences/Qiagen (Valencia, CA, USA) and tested for efficiency of gene silencing.

Techniques: shRNA, Transfection, Staining